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Z Series Coulter Counter

Precision, speed and versatility

Accurately count cells or particles (1-120 microns) with the Electrical Zone Method (EMZ) known as the Coulter Principle.

Features

Count and sizes cells or particles in the 1-120 micron size range with the Z Series Coulter Counter

For superior accuracy, speed, versatility and precision, the Z1 Single and dual-threshold models use the Coulter Principle – the accepted reference method for cell counting and sizing. Standard apertures are available in five sizes. Ampoule insertable apertures may be used for very small sample volumes.

Both Z1 single and dual threshold models use the Coulter Principle (Electrical Sensing Zone method) for counting cells or particles in the 1-120 micron size range.

Product Features

  • Mercury-free
  • Absolute cell counts or concentration
  • Single-button calibration
  • Compact
  • Certified to ISO 9001 by NSAI Quality Assurance
  • Technology defined by the International Standard (ISO 13319:2000: Determination of particle size distributions — Electrical Sensing Zone method)
  • Listed by ETL Testing Laboratory
  • Store up-to five analyses settings (i.e., profiles)
  • Operator-selectable size settings
Z1 COULTER COUNTER
  • Z1 Single-threshold model for rapid total cell count
  • Set one size threshold for counting cells equal to or larger than the established setting. The single-threshold model is ideal as a fully-automated system for rapid enumeration of relatively uniform tissue culture and blood cells from most any species.
  • Z1 Dual-threshold model for working with platelets or numerous cell types of variable sizes
  • Count cells or particles in three regions
  • Equal to-or-above the operator-selected lower size
  • Above the upper size-setting
  • Between the two size selections
The Z2 COULTER COUNTER for added capability of size distribution of cell population
  • Like the Z1, this instrument uses the accepted Coulter Principal for cell counting and sizing. Additional capabilities include-
  • Size distribution of a cell population
  • Entire size distribution graph, size statistics and counts between user-selected areas of the graph
  • Cumulative count and number % above and below a size determined by the cursor positioned on the graph
  • Average counts and channelized data from up-to 10 consecutive analyses with the Z2
  • Count and size more cells for smoother averaged size distribution and greater statistical confidence in results.

Data Acquisition Software

Windows-based AccuComp® software is available for quick and accurate calculation and display of Z2 sample data statistics.

AccuComp Features

  • Size distribution statistics
  • Data archiving
  • Size trend analysis
  • Result overlays
  • Averages, graphs and tables
  • Customizable report
  • Data import/export capability.

The Coulter Principle

While under contract to the United States Navy in the late 1940s, Wallace H. Coulter developed a technology for counting and sizing particles using impedance measurements. The technology was principally developed to count blood cells quickly by measuring the changes in electrical conductance as cells suspended in a conductive fluid passed through a small orifice. Presently, over 98% of automated cell counters incorporate this technology, which is referred to as the Coulter Principle. In the past seventy-five years, the technology has also been used to characterize thousands of different industrial particulate materials.

Beckman Coulter instrument systems which utilize this principle are called COULTER COUNTER® instruments. Drugs, pigments, fillers, toners, foods, abrasives, explosives, clay, minerals, construction materials, coating materials, metals, filter materials, and many other sample types have all been analyzed using the Coulter Principle. This method can be used to measure any particulate material that can be suspended in an electrolyte solution. Particles as small as 0.4 µm and as large as 1600 µm in diameter can routinely be measured. Over 8,000 references to the uses of this technology have been documented.

In a COULTER COUNTER instrument, a tube with a small aperture on the wall is immersed into a beaker that contains particles suspended in a low-concentration electrolyte. Two electrodes, one inside the aperture tube and one outside the tube but inside the beaker, are placed and a current path is provided by the electrolyte when an electric field is applied (Figure 1). The impedance between the electrodes is then measured. The aperture creates what is called a "sensing zone". Particles in low concentration, suspended in the electrolyte, can be counted by passing them through the aperture. As a particle passes through the aperture, a volume of electrolyte equivalent to the immersed volume of the particle is displaced from the sensing zone. This causes a short-term change in the impedance across the aperture. This change can be measured as a voltage pulse or a current pulse. The pulse height is proportional to the volume of the sensed particle. If constant particle density is assumed, the pulse height is also proportional to the particle mass. This technology is also referred to as aperture technology.

Using count- and pulse-height analyzer circuits, the number and volume of each particle passing through the aperture can be measured. If the volume of liquid passing through the aperture can be precisely controlled and measured, the sample concentration can also be determined. In modern COULTER COUNTER instruments, such as the Multisizer™ 3 and 4, particle counter and sizing instruments, pulses are digitized and saved with several key parameters that describe each pulse such as pulse height, pulse width, time stamp, pulse area, etc. These parameters enable the instrument to better discriminate between noise and real pulses as well as between normal and distorted pulses due to various reasons when particles pass through the aperture. Saved pulses can be used to monitor sample changes over the measurement time period should pulses be arranged in time sequence. In practice, particle volume is often represented in terms of equivalent spherical diameter. The measured particle volume (or size) can be then used to obtain particle size distribution.

With counting and sizing rates of up to 10,000 particles per second, it takes less than one minute to perform a typical measurement with a COULTER COUNTER instrument. The accuracy of size measurements can be better than 1%. Aperture size typically ranges from 20-2000 µm. Each aperture can be used to measure particles within a size range of 2 to 80% of nominal diameter. Therefore, an overall particle size-range of 0.4-1600 µm is feasible. However, the ability of the technology to analyze particles is limited to those particles that can be suitably suspended in an electrolyte solution. The upper limit therefore may be 500 µm for sand but only 75 µm for tungsten carbide particles. Moreover, the lower size limit is restricted by electronic noise generated mainly within the aperture itself. The selection of the most suitable aperture size is dependent upon the particles to be measured. If the sample to be measured is composed of particles largely within a 30:1 diameter size range, the most suitable aperture can be chosen. For example, a 30 µm aperture can measure particles from about 0.6 to 18 µm in diameter. A 140 µm aperture can measure particles from about 2.8 to 84 µm. If the particles to be measured cover a wider range than a single aperture can measure, two or more apertures must be used and the test results can be overlapped to provide a complete particle size distribution.

Highest Resolution for Particle Size Analysis

During the Coulter Principle measurement, as a particle passes through the sensing zone when the liquid is drawn from the container, a volume of the electrolyte equivalent to the immersed volume of the particle is displaced from the sensing zone. This causes a short-term change in the resistance across the aperture. This resistance change can be measured either as a voltage or current pulse. By measuring the number of pulses and their amplitudes, one can obtain information about the number of particles and the volume of each individual particle. 

The number of pulses detected during measurement is the number of particles measured, and the amplitude of the pulse is proportional to the volume of the particle. Because this is a single-particle measurement process, it yields the highest resolution that any particle characterization technique can achieve. The particle diameter can be determined at the resolution of voltage or current measurement which can be very accurately using current electronics technology. The distribution amplitude can be determined to the accuracy of a single particle.

The advantages of such high resolution are multiple with the most obvious being the capability to display details of a particle size distribution. In a particle size distribution measurement, typically each distribution, whether displayed cumulatively or differentially, is composed of a few-hundred data points in a pre-set size range. Each data point is called a bin. Since every particle is measured, each bin is a collection of particles in a given size range. Depending on the distribution broadness, the total size range can be reset to a finer division, therefore showing the distribution details (i.e., each bin can be pre-set to cover a smaller size range).

Other advantages include fine differential between two particles and more accurate statistic values calculated from the distribution. The figures below show a sample measured using the Beckman Coulter Multisizer 4 and displayed in different size ranges. The pulse data was resorted into a finer set of bins in the right figure in which more detail of the distribution is displayed.

Digital Pulse Process

In Coulter Principle instrumentation, the change in electric resistance due to passages of particles through the aperture is determined using fast electronic circuitry. Detected signals are instantaneously digitized at a rate of a few-million times per second into digital signals. The digital signal is then recorded for every pulse in the form of pulse parameters (i.e., timing, height, width of pulses, etc.). As most measurements seek to obtain particle counting or size distribution, the recorded pulse height is converted to particle size using the calibration constant and placed into one of the pre-set size bins. Particle size distribution and counting are the cumulative result of all pulses measured. All recorded pulse parameters are still available for purposes other than standard, full-range particle size distribution. These parameters can be subtracted or sorted (i.e., reprocessed differently based on specific applications). For example, if an operator seeks to have a zoom-in size distribution showing every detail of the distribution, then a narrower size range can be selected and all pulses can be sorted and placed into the new set of finer bins. Another example is found when pulse heights (or sizes) are sorted in a time sequence (for samples of narrow size-distribution) t monitor sample change during measurement. Still another example is using one plot pulse height as a function of pulse width to find information of particle shape.

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