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Оборудование

Beckman Coulter предлагает широкий ассортимент инновационных клеточных сортеров и проточных цитометров для расширения возможностей вашей клинической или научной лаборатории.

Navios

Proven reliability and precision for confident treatment decisions

Get the fast answers you need for timely, confident treatment decisions with the proven reliability and precision of Beckman Coulter’s Navios Tetra system for simultaneous identification and enumeration of T, B and NK lymphocytes in whole blood.

Navios is equipped with electronics that provide accurate and efficient digital signal processing at high event rates over a wide dynamic range of fluorescence intensities. These benefits are packaged in a compact analyzer that delivers stable performance over long periods of time and across a wide range of operating temperatures.

Now available for the Navios flow cytometer, Navios Tetra delivers an easy-to-use solution for multicolor flow cytometric analysis of lymphocyte subsets as well as CD4+ and CD8+ T cell subsets ratios.

Features



Accurate, automated and available on Navios

Power through your routine testing workload when you run on Navios with the innovative cluster tracking and absolute count robustness of Navios Tetra.

Fully automated set-up

  • Use Flow Check Pro and Flow Set Pro Fluorospheres for fast automated set up

Faster sample processing

  • Ready to use, pre-optimized reagents make sample prep easier than ever
  • Improve lab safety with reduced sample handling with no wash protocol
  • Streamline data entry and reporting with bi-directional LIS connectivity*

    *When used with Data Innovations Instrument Manager 8.05-8.12

Single platform standardization

  • Automated software algorithms define lymphocyte gate in three dimensions
  • Patient panel reports summarize T, B and NK results and internal reliability checks
  • Run-time reports highlight pertinent results

On-board quality control tracking

  • Process controls including low level CD4 counts
  • Built-in reliability and intra-panel checks
  • Sample flagging with insufficient T-cells

Performance

Optics in action

The Navios offers up-to four solid state, independently-focused diode lasers with an innovative steering design mechanism for optimal excitation at the flow cell and reliable high-quality results. Emitted energy is delivered by fiber optic technology to the patented boulevard using 18-degree reflective optics resulting in greater light collection efficiency for better resolve data, especially dim markers. The boulevard is optimized to offer application flexibility with accessible plug-and-play filters and he collection of tandem dyes.

Electronics explained

The Navios maintains a high yield of processed signals, greater than 90 per cent–even at 25,000 events per second.

  • 20 bit resolution
  • 1,048,576 channel display
  • More channels of resolution to separate even the dimmest of populations
  • Excellent accuracy and resolution for dim stains and quantitation

Patented electronics design ensures fast, accurate measurement of signals over a wide range of events.

  • Higher bits of information
  • Baseline restoration
  • Fast signal processing
  • Minimize data loss

High-yield electronics with fast pulse recovery at high speeds, the Navios electronic system minimizes linearity errors and adjusts for temperature variability.

  • Sensitivity of dim particles
  • Minimize dead time

The Navios electronic system has a self-correcting temperature regulation system.

  • Adjusts for temperature variability
  • Minimizes linearity errors based on temperature variations

Specifications

OPTICS

Lasers

LASERS/POWER OUTPUT

  • Blue Solid State Diode: 488nm, 22mW laser output
  • Red Solid State Diode: 638nm, 25mW laser output
  • Violet Solid State Diode: 405nm, 40mW laser output

CONFIGURATION

  • 125μm spatially separated beam spots

MINIMUM LASER POWER AT FLOW CELL

  • Blue: > 20mW
  • Red: > 20mW
  • Violet: > 30mW**
Flow Cell

150 x 460μm rectangular quartz

Collection Optics

Gel coupled 1.2 NA lens

Optical Filters

Easily interchangeable optical filters
Optimal 18-degree reflective optics for minimal light loss

Detector Filters

Forward Scatter: 488/10
Blue Laser: 525/40, 575/30, 620/30, 675/20**, 695/30, 755LP
Dyes: FITC, PE, ECD, PC5 or PEC5.5, PECy7 Red Laser: 660/20, 725/20, 755 LP
Dyes: APC or Alexa Fluor† 647, APCAlexa Fluor 700, APCCy7, APCAlexa Fluor 750
Violet Laser: **450/50, 550/40
Dyes: Pacific Blue†, Pacific Orange†, Krome Orange

Detectors

FORWARD SCATTER DETECTOR

  • Fourier design providing up to 3 measurements of forward angle

SIDE SCATTER DETECTOR

  • Independently focused high performance photodiode with electronic attenuation

FLUORESCENCE DETECTORS

  • FL1-FL10 Fluorescent Detectors (7-10 optional**)

SAMPLE PROCESSING

Flow Rates

Continuous pressure is applied to the sample tube based on user selected flow rates: Low Medium and High

Sheath Consumption

Acquisition: 780mL/hour
Carryover: <0.1%
Compatibility: 12x75mm tubes

Acquisition Modes

32 tube Multi Carousel Loader (MCL)
Single tube sampling mode
Automated work list acquisition
Manual work list mode

Mixing

The MCL patented design vortexes each tube individually before sample acquisition

Barcode Reading

Carousel number, tube location and tube barcode

Biosafety

Biohazard contained wash station thoroughly rinses sample probe

Fluidics

10L IsoFlow External Sheath Container
20L Waste Container
1.5L FlowClean Cleaning Fluid Tank
1.5L Internal Sheath Tank

SIGNAL PROCESSING

Flow Rates

Dynamic Range: 20-bit data acquisition
Workstation Resolution: 1,048,576 channels
Digital Sampling Rate: 40MHz
Digital Accuracy: <5% error
Parameters:

  • Five different signals available from each detector: Integral linear and logarithmic, Peak linear and logarithmic and True Time of Flight linear
  • Time, Ratio
  • Selection of up to 62 parameters

** Optionally available depending on upgraded system configuration
*** Optional filter included
† Alexa Fluor, Pacific Blue, and Pacific Orange are registered trademarks of Molecular Probes, Inc.
†† Pentium Core is a trademark of the Intel Corporation.
These characteristics can be influenced by a number of factors relating to instrument setup, sample type, number of parameters selected, protocol definition and number of events acquired. Refer to Instrument Instructions for Use for more information on Performance Characteristics.

Training Courses

File/Info Title Ref. Number
Excitation Emission & Filters
Filter Selection
Flow Applications
Flow Compensation
Flow Principles
Navios Customer Training

Videos

  • Navios - Electronics Explained

    Click to play  

    Navios is optimized for unparalleled signal to noise resolution in all parameters and its quick and easy setup of automated walk-away processing improves your workflow while its remote diagnostics minimize downtime and allow you to maximize lab...

  • Navios - Optics in Action

    Click to play  

    The Navios Flow Cytometer is a clinical system that delivers analytical excellence by coupling extraordinary sensitivity, resolution and dynamic range with high-speed data collection. With superior detection capabilities, the Navios provides...
  • VersaComp Antibody Capture Beads - Perfect Compensation

    Click to play  

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    Navios™, 10 colors, 3 lasers (5+3+2 configuration)

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    B47903

    Navios™, 6 colors, 2 lasers (5 + 1 configuration)

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    Request Quote

    B47904

    Navios™, 8 colors, 2 lasers (5 + 3 configuration)

    8 colors, 2 lasers (5 + 3 configuration)

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    775213

    SWRE KIT,NAVIOS TETRA SOFTWARE V1.1 NEW USER

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    CE marked for 10 color in-vitro diagnostic use.
    In U.S., Navios is intended for use as an in vitro diagnostic device for immunophenotyping with Navios tetra software and CYTO-STAT tetraCHROME reagents. All other uses are for research use only.
    Kaluza Questions & Concerns
    RNACleanXP Certificate of Analysis
    CleanSeq Certificate of Analysis
    AmpureXP Certificate of Analysis
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